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Tuesday 22 October 2013

Gene Copy Numbers Implicated in Complex Diseases


By duplicating them in zebrafish, very rare and precise duplications and deletions in the human genome have been connected by Duke researchers to their complex disease consequences.
The findings are based on detailed studies of five people missing a small fragment of their genome and suffering from a mysterious syndrome of craniofacial features, visual anomalies and developmental delays. When those patient observations were coupled to analyses of the anatomical defects in genetically altered zebrafish embryos, the researchers were able to identify the contribution specific genes made to the pathology, demonstrating a powerful tool that can now be applied to unraveling many other complex and rare human genetic conditions. The findings are broadly important for human genetic disorders because copy-number variants (CNVs) -- fragments of the genome that are either missing or existing in extra copies -- are quite common in the genome. But their precise contribution to diseases has been difficult to determine because CNVs can affect the function of many genes simultaneously. "Because a CNV can perturb many genes, it is difficult to know which of them is responsible," said Nicholas Katsanis, a professor of cell biology who directs the Center for Human Disease Modeling and the Task Force for Neonatal Genomics at Duke University. Last year, Katsanis and his colleagues found that they could trace recurrent copy-number variants and dissect the consequences of each perturbed gene to particular features in patients. The new study goes one step further by showing that they can also do this in more challenging cases, when CNVs differ in size from one individual to the next. In this case, "each person has his or her own private deletion or duplication," he said, with the potential to affect a different number of genes. The researchers showed that partially overlapping microdeletions found in the human patients include a region that contains three genes. By manipulating those genes in zebrafish, first one at a time and then in combination, they were able to connect the genes to specific features of the human syndrome. One of the study's first authors, post-doctoral researcher Christelle Golzio, recalled what it was like to see the characteristics of those human patients reflected in their laboratory fish for the first time. "The zebrafish had coloboma," she said, referring to holes in the iris or other parts of of the eye seen in these patients. "It was clear cut." Similar to the patients with the deletions, the zebrafish embryos also had kidney defects, small heads and defects in the development of the skeleton of the face. "This is a faster, cheaper, more efficient method to study and decipher copy-number variants, and the zebrafish model looks pretty robust in terms of recapitulating what the physicians observe in people," Golzio said. The findings also show just how complicated the consequences of copy-number variants can be. In some instances, the abnormalities seen in both patients and the fish were tied to copy number changes to individual genes, as that gene's "dosage" varied. At the same time, other characteristics were only observed when those genetic aberrations were combined, a pattern that would be nearly impossible to see in studies of humans themselves. In principle, the researchers say they can now examine the role of copy-number variants in any human syndrome, so long as the condition is associated with features that are measurable in the fish. "We will need to study lots of CNVs to find the edges of our capabilities," Katsanis said. "As we add this layer of dissection and interpretation, we will have prediction, diagnosis and the beginnings of biological understanding." 
Source:Duke University.

 

 

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